MspI RFLP at 19cen–q12 identified by the anonymous DNA sequence pPM17.4 [D19S29]

RFLP identified by the anonymous DNA segment FZ IV E11 at 1p23 [HGM9 no.D1S87].

Sequence-specific DNA binding by the MspI DNA methyltransferase.

The MspI methyltransferase (M.MspI) recognizes the sequence CCGG and catalyzes the formation of 5-methylcytosine at the fist C-residue. We have investigated the sequence-specific DNA-binding properties of M.MspI under equilibrium conditions, using gel-mobility shift assays and DNasel footprinting. M.MspI binds to DNA in a sequence-specific manner either alone or in the presence of the normal me...

MspI RFLP in the human fumarylacetoacetate hydrolase (FAH) gene.

An MspI PCR-RFLP detecting a single nucleotide polymorphism at alpha-lactalbumin gene in goat

Alpha-lactalbumin (α-LA, LALBA) was strongly correlated with the nutritional value and the functional properties of whey and whey products. However, there are not many studies of goat LALBA gene and its polymorphisms in literary sources. In this paper, on the basis of PCR-SSCP and DNA sequencing, one M63868:g.1897T>C mutation in exon 3 at LALBA locus identified a Single Nucleotide Polymorphism ...

Donor gametes: anonymous or identified?

The practice of gamete donation has, until recently, been shrouded in secrecy. The stigma associated with infertility and, in particular, donor insemination has been the main factor contributing to this secrecy. Over the last 20 years, this secrecy and the anonymity of the gamete donors has been challenged. In the first instance, the challenge came from governments in some countries legislating...